biotin-labeled human antibody microarray (raybio biotech  (RayBiotech inc)

Journal: Biomolecules

Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

doi: 10.3390/biom13040602

Figure Lengend Snippet: Different microarray printing strategies: ( A ) Fabrication of PDA patterned CYTOP (perfluoropolymer) glass slides via photolithography approach (reprinted with permission from ), ( B ) a representative of various steps involved in inkjet printing process (taken from https://gesim-bioinstruments-microfluidics.com/microarray-printer/ ; Accessed on 12 December 2022), and ( C ) GLAD method used to fabricate silver nanorods on glass slides (reprinted with permission from ).

Article Snippet: In various studies, commercially available microarrays, such as the Explorer Antibody Microarray (ASB6000, Full Moon Biosystems, Sunnyvale, CA, USA) with 656 antibodies [ ], VaxArray Coronavirus SeroAssay kit (#VXCV-5100, InDevR, Boulder, CO, USA) [ ], Human Cytokine Antibody Microarray slides (AAH-CYT-G4000 kit, RayBiotech, Peachtree Corners, GA, USA) [ ], Biotin-labeled Human antibody microarray (RayBio Biotech, Guangzhou, China) [ ], Protein Microarray Slides (Grace Bio-Labs, Bend, OR, USA) [ ], HuProt human proteome microarrays (CDI laboratories, Mayaguez, PR, USA) [ ], SBC Mouse ceRNA microarray V1.0 (Shanghai Biotechnology Corporation, Shanghai, China) [ ], and Agilent Whole Mouse 44 K ver.

Techniques: Microarray

Journal: Biomolecules

Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

doi: 10.3390/biom13040602

Figure Lengend Snippet: Detection methods: ( A ) FRET based approach to detect target DNA. In presence of target DNA only, the existing complex of fluorescence dye (Cy3) and quencher (BHQ2) is disrupted and generates fluorescence signal (). ( B ) CL based enzymatic approach. This approach involves multiple steps: immunoreaction, HCR amplification, enzyme conjugation, and enzyme-CL (luminol p-iodophenol) substrate reaction (). ( C ) Colorimetric detection (use of streptavidin-coated gold nanoparticles) of isothermal DNA amplification. Interaction of biotin and streptavidin was explored (). ( D ) OIRD, a label-free detection method to study protein/antibody interactions with target molecules on a protein microarray chip ().

Article Snippet: In various studies, commercially available microarrays, such as the Explorer Antibody Microarray (ASB6000, Full Moon Biosystems, Sunnyvale, CA, USA) with 656 antibodies [ ], VaxArray Coronavirus SeroAssay kit (#VXCV-5100, InDevR, Boulder, CO, USA) [ ], Human Cytokine Antibody Microarray slides (AAH-CYT-G4000 kit, RayBiotech, Peachtree Corners, GA, USA) [ ], Biotin-labeled Human antibody microarray (RayBio Biotech, Guangzhou, China) [ ], Protein Microarray Slides (Grace Bio-Labs, Bend, OR, USA) [ ], HuProt human proteome microarrays (CDI laboratories, Mayaguez, PR, USA) [ ], SBC Mouse ceRNA microarray V1.0 (Shanghai Biotechnology Corporation, Shanghai, China) [ ], and Agilent Whole Mouse 44 K ver.

Techniques: Fluorescence, Amplification, Conjugation Assay, Microarray

Journal: Biomolecules

Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

doi: 10.3390/biom13040602

Figure Lengend Snippet: ( A ) A pictorial workflow presentation of hybrid tetrahedral DNA structured probe in conjugation with hybridization chain reaction (DTSP-HCR) concept used to distinguish single-base mismatches in DNA () and ( B ) an overview of acute tuberculosis (ATB) biomarker identification using a two-phase strategy (discovery phase and validation phase) (). Venn diagram and microarray chip visual analysis revealed the potential of 5 protein biomarkers to distinguish ATB and LTBI/HC. LTBI represents latent tuberculosis infection, and HC represents healthy control.

Article Snippet: In various studies, commercially available microarrays, such as the Explorer Antibody Microarray (ASB6000, Full Moon Biosystems, Sunnyvale, CA, USA) with 656 antibodies [ ], VaxArray Coronavirus SeroAssay kit (#VXCV-5100, InDevR, Boulder, CO, USA) [ ], Human Cytokine Antibody Microarray slides (AAH-CYT-G4000 kit, RayBiotech, Peachtree Corners, GA, USA) [ ], Biotin-labeled Human antibody microarray (RayBio Biotech, Guangzhou, China) [ ], Protein Microarray Slides (Grace Bio-Labs, Bend, OR, USA) [ ], HuProt human proteome microarrays (CDI laboratories, Mayaguez, PR, USA) [ ], SBC Mouse ceRNA microarray V1.0 (Shanghai Biotechnology Corporation, Shanghai, China) [ ], and Agilent Whole Mouse 44 K ver.

Techniques: Conjugation Assay, Hybridization, Biomarker Assay, Microarray, Infection

Journal: Biomolecules

Article Title: Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

doi: 10.3390/biom13040602

Figure Lengend Snippet: A generic workflow of the application of glycan microarray chip to efficiently profile enzyme activities and determine enzyme inhibitor IC 50 values ().

Article Snippet: In various studies, commercially available microarrays, such as the Explorer Antibody Microarray (ASB6000, Full Moon Biosystems, Sunnyvale, CA, USA) with 656 antibodies [ ], VaxArray Coronavirus SeroAssay kit (#VXCV-5100, InDevR, Boulder, CO, USA) [ ], Human Cytokine Antibody Microarray slides (AAH-CYT-G4000 kit, RayBiotech, Peachtree Corners, GA, USA) [ ], Biotin-labeled Human antibody microarray (RayBio Biotech, Guangzhou, China) [ ], Protein Microarray Slides (Grace Bio-Labs, Bend, OR, USA) [ ], HuProt human proteome microarrays (CDI laboratories, Mayaguez, PR, USA) [ ], SBC Mouse ceRNA microarray V1.0 (Shanghai Biotechnology Corporation, Shanghai, China) [ ], and Agilent Whole Mouse 44 K ver.

Techniques: Microarray